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应用逆转录－套式聚合酶链反应（ＲＴ－ｎＰＣＲ）法，对２６例散发性戊型肝炎（ＨＥ）患者发病后４～２８天收集的９１份粪便标本进行ＨＥＶＲＮＡ检测，并与同病期系列血清ＨＥＶＲＮＡ及ＡＬＴ水平作比较，以了解散发性ＨＥ患者的粪便排病毒规律。结果表明，患者粪便ＨＥＶＲＮＡ阳性率为４２．３％（１１／２６），与血清ＨＥＶＲＮＡ阳性率（５３．８％）相比无显著性差异（Ｐ＞０．０５）。粪便ＨＥＶＲＮＡ检出率在发病头５天内为７５％（３／４），随后即与血清ＡＬＴ水平一样出现明显下降，于发病后１６～２０天降至１９．２％（５／２６），最长一例持续阳性至发病后２３天。上述结果提示，ＨＥ患者的粪便排病毒主要发生在疾病的急性期早期，将其隔离期定为病后３周可能比较合理。
Totally 91 stool samples from 26 to 34 days after onset of sporadic HE were detected by reverse transcriptase-nested polymerase chain reaction (RT-nPCR) Series of serum HEVRNA and ALT levels were compared to understand the sporadic HE in patients with sporadic virus law. The results showed that the positive rate of HEV RNA in stool was 42.3% (11/26), which was not significantly different from the positive rate of HEV RNA in serum (53.8%) (P> 0.05). The detection rate of stool HEV RNA was 75% (3/4) in the first 5 days of onset, then decreased obviously as the level of serum ALT, and dropped to 19.2% (5/26) at 16-20 days after onset A case of persistent positive to 23 days after onset. These results suggest that HE patients with stool virus occurs mainly in the early stages of acute disease, the isolation period of 3 weeks after the disease may be more reasonable.