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目的缩小喉癌抑癌基因的寻找范围，探讨ｐ１６基因在喉癌发生中的作用。方法选择ｐ１６基因附近５个微卫星多态标记对６０例喉癌进行杂合性丢失和微卫星序列不稳定性分析。结果５个标记在喉癌中杂合性丢失频率均不高，最高仅达２３．１％，但２个标记的微卫星序列不稳定性的频率较高，其中一个标记微卫星序列不稳定性频率高达４６．１％。结论提示ｐ１６基因在喉癌的发生中不以缺失为主，在Ｄ９Ｓ１７５２附近可能存在参与喉癌演进的基因。
Objective To narrow the search range of tumor suppressor genes in laryngeal cancer and to explore the role of p16 gene in the pathogenesis of laryngeal cancer. Methods Fifty microsatellite loci adjacent to the p16 gene were selected to detect loss of heterozygosity and microsatellite instability in 60 laryngeal cancer patients. Results The frequency of loss of heterozygosity of all five markers was not high in laryngeal carcinoma, up to only 23.1%. However, the frequencies of microsatellite instability of two markers were higher than those of the other two markers. One of the markers was microsatellite instability Frequency up to 46.1%. The results suggest that p16 gene is not deleterious in the occurrence of laryngeal carcinoma, and there may exist genes involved in the evolution of laryngeal carcinoma near D9S1752.