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为深入探究中间砧影响苹果果实苹果酸代谢的机理,以SH40实生后代(代号53、111和236)为中间砧嫁接的‘天红2号’苹果树为试材,测定果实发育过程中苹果酸含量、相关代谢酶活性及基因相对表达量。结果表明:果实成熟时,以53号为中间砧嫁接的‘天红2号’果实苹果酸含量显著高于以111号为中间砧的。盛花后30、40、100~160 d,以53号为中间砧的果实中苹果酸脱氢酶(NAD-MDH)活性显著高于以111号为中间砧的;盛花后30、40和130 d,53号处理的磷酸烯醇式丙酮酸羧化酶(PEPC)活性显著高于111号处理的;盛花后100 d,以111号为中间砧的果实中苹果酸酶(NADP-ME)活性显著高于以53号为中间砧的。基因表达结果显示,盛花后100和160 d,以53号为中间砧的NAD-MDH基因相对表达量显著高于以111号为中间砧的,同时NADP-ME基因表达量也显著高于以111号为中间砧的;盛花后30和160 d,以53号为中间砧的PEPC基因相对表达量显著高于以111号为中间砧的。
In order to explore the mechanism of the effects of intermediate anvil on malic acid metabolism in apple fruit, the maize (Tianhong No.2) apple grafted with SH40 progeny (code 53, 111 and 236) was used as test material to determine the content of malic acid Content, relative metabolic enzyme activity and relative gene expression. The results showed that malic acid content of ’Tianhong No.2’ grafted with No.53 was significantly higher than that of No.111 when the fruit matured. Maize dehydrogenase (NAD-MDH) activity was significantly higher than that of No. 111 as an anvil at 30, 40, 100-160 d after flowering; (PEPC) activity of No.53 treatment was significantly higher than that of No.1111 treatment. The activity of NADP-ME in the fruit of No.111 dwarfing was the highest Higher than No. 53 as anvil. The results of gene expression showed that the relative expression level of NAD-MDH was significantly higher than that of No. 111 at 100 and 160 days after flowering, and the expression level of NADP-ME gene was also significantly higher than that of 111 No. 33 was the middle anvil. After 30 and 160 days of full bloom, the relative expression level of PEPC with No. 53 as the anvil was significantly higher than that with No. 111 as the intermediate anvil.