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目的:建立竹柏叶中穗花双黄酮含量测定方法。方法:采用高效液相色谱法,色谱条件为:Agilent C18色谱柱(150 mm×4.6 mm,5μm);乙腈-0.05%磷酸水液为流动相,梯度洗脱(时间0~30 min,乙腈15%~100%);流速为1.0 mL.min-1,检测波长为340 nm。结果:穗花双黄酮在0.013~0.130μg范围内线性关系良好(r=0.9999),平均加样回收率为96.34%,RSD为1.4%(n=6)。结论:本法操作简便,结果准确,重现性好,可用于竹柏叶中穗花双黄酮成分的含量测定,并为其开发应用提供基础。
Objective: To establish a method for the determination of biflavonoids in spike flowers of P. bracteatum. Methods: HPLC method was used. The chromatographic conditions were as follows: Agilent C18 column (150 mm×4.6 mm, 5 μm); acetonitrile-0.05% phosphoric acid aqueous solution as mobile phase, gradient elution (time 0-30 min, acetonitrile 15 %~100%); flow rate 1.0 mL.min-1, detection wavelength 340 nm. RESULTS: The linearity of spiked flavonoids in the range of 0.013 to 0.130 μg was good (r=0.9999). The average recovery was 96.34% and the RSD was 1.4% (n=6). Conclusion: This method is simple, accurate and reproducible. It can be used to determine the content of biflavonoids in the flower of P. bracteatum and provide basis for its development and application.