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White Flammulina velutipes strains FM0 F21,F10 and F3-W were selected as the parental strains in a single-spore cross-breeding experiment.Thirty basidiospore-derived monokaryons were selected randomly from each parental strain,and mating was carried out according to the mating groups:FM×F21,FM×F3-W and F3-W×F10.Integrative analysis revealed that hybrid progenies from the FM×F21 mating group exhibited the highest mating rate (79%).However,only 59.2% of these hybrids produced measurable fruit body yields compared with 82.6% and 84.4% of the hybrids generated from FM×F3-W and F10×F3-W crosses respectively,perhaps indicating that certain hybrid progeny were better adapted to the standard set of cultivation conditions adopted in the study.Mating rates for FM×F3-W and F10 x F3-W crosses were 28% and 42%,respectively indicating that the number of mating type genes may vary among the monokaryons constituting each of the three mating groups.Mycelial growth rates of most FM×F21 hybrid progeny ranged between 4.0～7.0 mm/d.Corresponding values for progeny from FM×F3-W and F10×F3-W crosses were 3.0～6.0 mm/d and 2.0～6.0 mm/d,respectively although a small number of dikaryons from these two mating groups exhibited much higher/lower growth rates.No correlation between mycelial growth rate and fruit body yield was evident for any of the three sets of hybrid progeny.Fruit bodies produced by all the hybrid progeny were white in color.
White Flammulina velutipes FM0 F21, F10 and F3-W were selected as the parental strains in a single-spore cross-breeding experiment. Thirty basidiospore-derived monokaryons were selected randomly from each parental strain, and mating was carried out according to the mating groups: FM × F21, FM × F3-W and F3-W × F10. Intergrative analysis revealed that hybrid progenies from the FM × F21 mating group exhibited the highest mating rate (79%). However, only 59.2% of these hybrids produced measurable fruit body yields compared with 82.6% and 84.4% of the hybrids generated from FM × F3-W and F10 × F3-W crosses respectively, perhaps indicating that certain hybrid progeny were better adapted to the standard set of cultivation conditions adopted in the study .Mating rates for FM × F3-W and F10 × F3-W crosses were 28% and 42%, respectively indicating that the number of mating type genes may vary among the monokaryons each each the three mating groups. Mycelial growth rates of most FM × F21 hybrid pr ogeny ranged between 4.0 and 7.0 mm / d. Corresponding values for progeny from FM × F3-W and F10 × F3-W crosses were 3.0-6.0 mm / d and 2.0-6.0 mm / d, respectively although a small number of dikaryons from these two mating groups show much higher / lower growth rates. No correlation between mycelial growth rate and fruit body yield was evident for any of the three sets of hybrid progeny. Fruit bodies produced by all the hybrid progeny were white in color.