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目的:研究新型棉酚衍生物Apog-ossypolone(ApoG2)在体外对前列腺癌LNCaP细胞的自噬作用。方法:采用MTT、AO染色、透射电镜、流式细胞术和免疫组化方法,研究ApoG2对前列腺癌LNCaP细胞的生长抑制及诱导自噬的作用。结果:当ApoG2浓度>2.5μg/mL时,对前列腺癌LNCaP细胞有明显的增殖抑制,且有时间与剂量依赖性,ApoG2作用LNCaP细胞72 h时的IC50值为4.43μg/mL。AO染色观察LNCaP细胞有自噬特征;透射电镜观察细胞内有自噬泡形成;流式细胞术检测细胞内出现大量酸性液泡细胞器(AVOs);免疫组化结果显示,ApoG2作用于LNCaP细胞后可以使细胞内Beclin 1的表达增高。结论:ApoG2在体外可以诱导前列腺癌LNCaP细胞的自噬,抑制前列腺癌LNCaP细胞的生长。
Objective: To study the autophagy effect of a novel gossypol derivative Apog-ossypolone (ApoG2) on prostate cancer LNCaP cells in vitro. Methods: MTT, AO staining, transmission electron microscopy, flow cytometry and immunohistochemistry were used to study the effect of ApoG2 on the growth of LNCaP cells and the induction of autophagy. Results: When ApoG2 concentration was> 2.5μg / mL, LNCaP cells showed significant proliferation inhibition in a time-and dose-dependent manner. The IC50 of ApoG2-treated LNCaP cells for 72h was 4.43μg / mL. Apoptosis of LNCaP cells was observed by AO staining. Autophagic vacuoles were observed by transmission electron microscopy. A large number of acidic vacuolar organelles (AVOs) were detected by flow cytometry. The results of immunohistochemistry showed that ApoG2 could act on LNCaP cells Beclin 1 expression in cells increased. Conclusion: ApoG2 can induce autophagy of prostate cancer LNCaP cells in vitro and inhibit the growth of prostate cancer LNCaP cells.