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植物名称:扎伊尔马兜铃(Aristolochia ele-gams Mast) 材料类别:嫩茎消毒后切成1cm长的切段。培养条件:基本培养基为MS附加BA、IAA和2,4-D等植物激素,培养温度25—30℃,每天照光10小时(光强为2000lux)。生长和分化情况:茎段在附加BA2、IAA0.2-0.4mg/l的MS培养基上或BA2、2,4-D0.2mg/l的MS培养基上,6天茎段开始膨大,7—12天可见透明的愈伤组织产生,诱导率为89—100％,随后又长出绿色瘤状愈伤组织,16天开始由瘤状愈伤组织产生丛芽,这些带芽愈伤组织可以切割、转接不断增殖下去。产生丛芽的最适激素配比浓度是BA2、IAA0.2mg/l,其次是BA2、2,4-D0.2mg/l,
Plant Name: Aristolochia ele-gams Mast Material Type: cut into 1cm long stalks after sterilization. Culture conditions: The basic medium for the MS with BA, IAA and 2,4-D and other plant hormones, culture temperature 25-30 ℃, light 10 hours a day (light intensity 2000lux). Growth and differentiation: Stem segments were MS medium supplemented with BA2, IAA0.2-0.4mg / l or MS medium BA2,2,4-D0.2mg / -12 days transparent callus can be seen, the induction rate of 89-100%, followed by the growth of green tumor-like callus, 16 days began to produce by the callus tumor buds, callus with these buds can Cutting, transfer continue to proliferate. The optimal concentration of hormone to produce bud budding is BA2, IAA0.2mg / l, followed by BA2,2,4-D0.2mg / l,