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谷胱甘肽 S 转移酶(GST)是参与解毒的酶类,分为α(为碱性)、μ(为中性)、π(为酸性)3类,与治疗癌症的耐药性有关。近年,已有很多关于肺癌与 GST 同工酶的研究,例如 Di Ilio 等从38例肺非肿瘤部位与肺肿瘤部位提出蛋白质,测定 GST 的酶活性,发现肺肿瘤部分明显增高;Pasquini 等用22例同样标本分析吸烟对 GST活性的影响,发现吸烟者肿瘤部位 GST 升高,而非肿瘤部位低下;Carmichael 等对10例同样标本进行酸性、中性和碱性的研究,发现肿瘤部或非肿瘤部酸性(π类)均增加;中性(μ类)在低分化型鳞癌及低分化型腺癌增加,大细胞癌和小细胞癌无变化或降低;碱性(α类)与肿瘤组织类型无关。Singh 等的5例标本中有4例 GST-π的酶活性增高。作者对67例肺癌患者的尸检、活检及手术所得的组织进行免疫组织化学分析,发现 GST-π。结果表
Glutathione S transferase (GST) is an enzyme involved in detoxification, and is classified into alpha (alkaline), mu (neutral), and pi (acidic) types and is related to the treatment of cancer resistance. In recent years, there have been many studies on lung cancer and GST isoenzymes. For example, Di Ilio et al. presented proteins from 38 non-tumor sites and lung tumor sites to determine the GST enzyme activity and found that lung tumors were significantly increased; Pasquini et al.22 The same sample was used to analyze the effect of cigarette smoking on GST activity. It was found that the GST in the tumor site increased, but not in the tumor site; Carmichael et al. performed acid, neutral, and alkaline studies on 10 of the same samples and found that the tumor was or not Increased acidity (π); Neutral (μ) increased in poorly differentiated squamous cell carcinoma and poorly differentiated adenocarcinoma, no change or decrease in large cell carcinoma and small cell carcinoma; Alkaline (α) and tumor tissues Type has nothing to do. Singh et al.5 showed elevated GST-π activity in 5 samples. The authors performed immunohistochemical analysis on the autopsy, biopsy and tissue obtained from 67 patients with lung cancer and found GST-π. Result table