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目的:研究人LAIR-1/CD305启动子及其5′上游调控序列。方法:通过检索NCBI中的人类基因组数据库,获得LAIR-1的转录本序列及翻译起始位点上游2500bp的序列。利用Promoter2.0等软件预测LAIR-1的启动子序列,然后利用MatInspector等软件对启动子序列的转录因子结合位点和启动子功能模块进行预测。结果:LAIR-1基因的核心启动子区位于翻译起始密码子上游的600～200bp区域内。在转录调控区发现了一些重要的转录因子结合位点,并预测到13种启动子功能模块。结论:LAIR-1基因的表达可能受到多种转录因子和5′端上游调控序列的调控。
AIM: To investigate the human LAIR-1 / CD305 promoter and its 5 ’upstream regulatory sequence. Methods: The transcript sequence of LAIR-1 and the sequence of 2500 bp upstream of the translation initiation site were obtained by searching the human genome database in NCBI. The Promoter2.0 software was used to predict the promoter sequence of LAIR-1, and then the software was used to predict the transcription factor binding site and promoter function of the promoter sequence using software such as MatInspector. RESULTS: The core promoter region of the LAIR-1 gene was located in the 600-200 bp region upstream of the translation initiation codon. A number of important transcription factor binding sites were found in the transcriptional regulatory region and 13 promoter functional modules were predicted. Conclusion: The expression of LAIR-1 gene may be regulated by many transcription factors and 5 ’upstream regulatory sequences.