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目的:探索陈旧组织标本在制作教学切片中的方法。方法:将陈旧组织先用碱盐、PBS、TritonX-100、Lougl’s液、硫酸铁铵预处理再进行HE染色。结果:本方法在检验陈旧组织切片中,组织着色均匀,细胞核、核膜及核内结构清晰,核浆着色对比明显。结论:运用此方法对目前教学切片来源不足具有重要实用价值,值得推广应用。
Objective: To explore the method of making old teaching specimens in making teaching slices. Methods: The stained old tissue was pretreated with alkali salt, PBS, TritonX-100, Lougl’s solution and ammonium ferric sulfate before HE staining. Results: The method was used to check the stained old tissue sections, and the tissue was stained homogeneously. The structure of nucleus, nucleus and nucleus was clear and the contrast of nucleus pulps was obvious. Conclusion: This method has important practical value for the lack of source of teaching slides at present, which is worth popularizing and applying.