【摘 要】
：
Aim: To investigate the effect of peroxisome proliferator-activated receptor gamma (PPARγ) activation on the growth of rat hepatic stellate cells (HSC) and tra
【机 构】
：
Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangz
论文部分内容阅读
Aim: To investigate the effect of peroxisome proliferator-activated receptor gamma (PPARγ) activation on the growth of rat hepatic stellate cells (HSC) and transforming growth factor beta (TGF-β-induced connective tissue growth factor (CTGF) expression. Methods: After being treated with various amounts of the PPARγ natural ligand 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2) or synthetic ligand GW7845, the status of HSC proliferation and apoptosis were detected by MTT assay and flow cytometry. Furthermore, HSC were treated with PPARγ-specific antagonist GW9662 prior to the addition of 15-d-PGJ2 or GW7845 and were subsequently stimulated with TGF-β1. The mRNA and protein levels of CTGF expression were detected by semi-quantitative RT-PCR and Weste blotting analysis. Morphological changes in the HSC were observed by electron microscopy. Results: 15-d-PGJ2 and GW7845 markedly inhibited HSC proliferation and induced cell apoptosis in a dose-dependent manner. Furthermore, PPARγligands significantly suppressed TGF-β1-induced CTGF expression (at both transcriptional and post-transcriptional levels) in HSC, and the inhibitory effect was dramatically, if not completely, abolished by pretreatment with GW9662, suggesting that the inhibition was indeed mediated by PPARγ. Moreover, morphological observation revealed that PPARγactivation caused obvious changes of HSC from activated to quiescent phenotype. Conclusion: The PPARγligand has a potent inhibitory effect on the growth of HSC and TGF-β1-induced CTGF expression, which makes it a potential antifibrotic candidate for the treatment and prevention of hepatic fibrosis.
其他文献
品种更替是小麦生产发展的必要手段和必然趋势，在品种更替过程中，一些性状表现不符合生产实践需要的品种得到了更换，同时，一些特征性状生产性能优秀的品种得到了更新。品种资源生态型作为作物生态研究的一个重要内容，同时，小麦品种生态型是小麦生态研究的基础。近年来，伴随着作物气候生态的研究的深入，品种资源生态型演化作为分析小麦生态规律和研究小麦品种的生态适应性的一个重要内容，也越来越受到重视和关注。探究小麦生
Aim: To investigate the protective effect of steroidal saponins from Anemarrhena asphodeloides (ATS) on ovariectomy (OVX)-induced bone loss. Methods: Sprague-Da
Soybean (Glycine max L.Merr.)is oneof the most prominent legume crops playingsignificant role in worlds food security.Water stress is one of the mostsevere cons
Aim: To comparatively study the pharmacological profiles of 3-methyl-3-azabicyclo (3,3,1)nonanyl-9-α-yl-α-cyclopentyl-α-phenyl-α-glycolate (phencynonate hyd
为了加快作物遗传改良的进程,将外源目的基因导入植物体,并筛选出极少量的转化细胞,一套高效安全的选择方法极为重要。以往的筛选标记因为存在于转基因植物中时,其抗生素或除
随着油菜用途的扩展，其种植面积逐年扩大，而油菜存在油菜黑腐病等多种危害严重的病害尚未得到根治，常规使用的化学农药防治方法则又极易造成环境污染。Harpin是自然界中特定的细菌性植物病原菌产生的一种由hrp基因编码的蛋白质。尽管Harpin本身不具有任何杀菌活性，但它能诱导植物产生一种复杂的自然防御反应，抵御真菌、细菌和病毒病害。因此，在油菜中导入十字花科植物黑腐病菌(Xcc)hrpW基因，使油菜自
In non-excitable cells such as T lymphocytes,hepatocytes,mast cells,endothelia and epithelia,the major pathway for calcium(Ca2+)entry is through store-operated
通过发芽试验，根据相对发芽率，筛选不同耐NaCl玉米自交系；采用砂培的试验方法，研究了不同浓度NaCl处理(对照不含NaCl)下两个具有不同耐NaCl基因型的玉米自交系形态及生理特性的差
“混制1号”(绿三A/mc526)系安徽省农科院绿色食品工程所育成的中籼杂交稻,其父本携带苯达松(除草剂)敏感基因,在杂交制种的抽穗期喷施苯达松完成授粉后植株枯死,基本不会自交结实;母本未携带该基因,喷药后仍能异交结实和正常成熟,且与父本生育期相近,因此该组合制种可将父母本种子按一定比例混合后进行人工或机械播植,并能获得较高的制种产量和质量合格的杂交种。混播制种与父母本分别播种按一定行比栽插的常规
Aim: To construct an HEK293 cell line stably expressing human dopamine D1 receptor (D1R). Methods: cDNA was amplified by RT-PCR using total RNA from human embry