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目的:探讨补肾活血法对SD大鼠骨折愈合过程中结缔组织生长因子表达的影响。方法:清洁级SD大鼠40只,6月龄,随机分为对照组(A组)及补肾活血汤组(B组),建立骨折模型。A组大鼠灌胃蒸馏水,B组大鼠灌胃补肾活血汤溶液,灌胃给药容积为10 m L/kg。造模后第3、7、14、21天,每组随机选取5只大鼠,切取骨痂标本,采用RT-PCR法和Western-blot法分别检测结缔组织生长因子(CTGF)的m RNA和蛋白质的表达。结果:结果显示,A组术后3天、7天及14天的CTGF m RNA和蛋白的表达逐渐增强,至21天表达下降,与术后14天比较,差异有统计学意义(P<0.05)。术后3天,B组CTGF m RNA和蛋白无表达,术后7天、14天及21天,B组的CTGF m RNA和蛋白的表达逐渐增强,术后21天与术后14天比较,差异有统计学意义(P<0.05)。术后21天,B组较A组的CTGF m RNA和蛋白的表达均显著升高,差异有统计学意义(P<0.05)。结论:补肾活血能够促进骨折愈合过程中结缔组织生长因子蛋白和m RNA表达,从而促进骨折愈合。
Objective: To investigate the effect of tonifying kidney and promoting blood flow on the expression of connective tissue growth factor in fracture healing of SD rats. Methods: Forty clean-grade SD rats were randomly divided into control group (A group) and Bushen Huoxue decoction group (B group) at the age of 6 months. Fracture models were established. Rats in group A were fed with distilled water. Rats in group B were fed with Bushen Huoxue Decoction solution in a volume of 10 m L / kg. On the 3rd, 7th, 14th and 21st day after modeling, 5 rats were randomly selected from each group. The callus samples were obtained and the mRNA and protein expression of connective tissue growth factor (CTGF) were detected by RT-PCR and Western- Protein expression. Results: The expression of CTGF m RNA and protein in group A at 3 days, 7 days and 14 days after operation increased gradually and decreased to 21 days after operation compared with 14 days after operation (P <0.05 ). Three days after operation, CTGF m RNA and protein were not expressed in group B, and the expression of CTGF m RNA and protein in group B was gradually increased at 7 days, 14 days and 21 days after operation. After 21 days and 14 days after operation, The difference was statistically significant (P <0.05). At 21 days after operation, the expression of CTGF m RNA and protein in group B was significantly higher than that in group A, the difference was statistically significant (P <0.05). Conclusion: Bushenhuoxue can promote the expression of connective tissue growth factor protein and m RNA during fracture healing and promote fracture healing.