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用免疫酶组织化学Sternberger过氧化物酶-抗过氧化物酶 (PAP) 方法,研究了鼠胚和新生大鼠心脏心房肽免疫反应颗粒的发生和分布。鼠胚龄以大鼠受孕天数计算。下午9～10h将雌、雄大鼠同笼,次日清晨检查雌性大鼠阴道中出现精子,定为大鼠受孕第1d。在大鼠受孕第12～20d每天下午取材;新生大鼠为出生后6h内取材。共用鼠胚和新生大鼠41只,各天所用鼠胚数量为3～5只。所
Immunohistochemical Sternberger peroxidase - anti-peroxidase (PAP) method was used to study the occurrence and distribution of atrial natriuretic peptide immunoreactive particles in rat embryos and neonatal rat hearts. Rat embryonic age to calculate the number of days of pregnancy in rats. 9 ~ 10h afternoon female and male rats in the same cage, the next morning check sperm in the vagina of female rats, as the first day of pregnancy in rats. Twelve to twenty days after conception in rats were harvested every afternoon; newborn rats were harvested within 6 hours after birth. There were 41 embryos and newborn rats in total. The number of mouse embryos used in each day was 3 ~ 5. The