论文部分内容阅读
为深入调查中华按蚊的种内变异,本人利用1978年由中国医学科学院寄生虫病研究所引进的上海中华按蚊室内品系的四龄幼虫制备,观察了大量唾腺染色体标本。方法是:在偏低温(24～26℃)条件下给予充足饲料培养幼虫,选择肥大的四龄幼虫,在5％醋酸中解剖出唾腺,再用50％醋酸固定1～2min,然后用2％地衣红(由50％醋酸和50％乳酸配制)染色。高倍光学显微镜观察,油镜照相。
In order to further investigate the intraspecific variation of Anopheles sinensis, I used a large number of salivary gland chromosome specimens from the fourth instar larvae of the Chinese Anopheles sinensis indoor strain introduced by the Institute of Parasitic Diseases, Chinese Academy of Medical Sciences in 1978. The method comprises the following steps of: feeding larvae with sufficient feed under the condition of low temperature (24-26 DEG C), selecting the fourth instar larvae of hypertrophy, dissecting the salivary glands in 5% acetic acid, fixing the salivary glands in 50% acetic acid for 1 to 2 minutes and then using 2 % Lichen red (formulated with 50% acetic acid and 50% lactic acid). High magnification optical microscope observation, oil mirror photography.