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目的:探讨低温真空甲醛的杀菌效果,并于氧化法传统甲醛熏箱进行比较。方法:试验组采用自研的140L低温真空甲醛灭菌箱,将被灭菌器械装入纸塑包装袋竖放在箱内,采用其程控灭菌程序;对照组采用氧化法传统甲醛熏箱,被灭菌器械裸放于箱内隔板上,采用高锰酸钾加40%甲醛的氧化法,密闭12h。两组灭菌效果监测均采用常规和PVD加强型“枯草杆菌芽孢菌管”。结果:试验组的常规和PVD加强型“枯草杆菌芽孢菌管”全部杀灭,对照组常规和PVD加强型“枯草杆菌芽孢菌管”全部未被杀灭。结论:氧化法传统甲醛熏箱穿透力差;低温真空甲醛灭菌精确控制了甲醛剂量、温度、湿度、作用时间、作用压力,真空状态下的甲醛气体容易穿透器械包的中心或导管的内部,能杀灭所有微生物包括芽孢,适合对细长管道、多腔隙电动力器械、电子仪器和精密光学仪器的灭菌。
Objective: To investigate the bactericidal effect of vacuum formaldehyde at low temperature, and to compare the traditional formaldehyde fumigation box by oxidation method. Methods: The experimental group was self-developed 140L vacuum formaldehyde for vacuum sterilization box, the sterilized instruments were placed in paper-plastic packaging bags placed in the box, using its program-controlled sterilization procedures; the control group using the traditional method of oxidation of formaldehyde, Sterilized instruments were placed naked in the box partition, the use of potassium permanganate plus 40% formaldehyde oxidation, closed 12h. Sterilization monitoring of both groups were performed using conventional and PVD-enhanced Bacillus subtilis tubes. Results: All the conventional and PVD-enhanced Bacillus subtilis tubes in the experimental group were killed, while the control and PVD-enhanced Bacillus subtilis tubes were all killed. CONCLUSION: The traditional method of formaldehyde fumigation with poor penetrability is poor. Low-temperature vacuum formaldehyde sterilization precisely controls the dosage, temperature, humidity, action time and pressure of formaldehyde, and the formaldehyde gas in vacuum easily penetrates the center of the instrument package or the catheter Internal, can kill all microorganisms including spores, suitable for slender pipes, multi-cavity electrodynamic devices, electronic instruments and precision optical instruments sterilization.