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作者用引物Y_3、Y_4和DNA聚合酶链式反应(PCR)作微量人类血液(痕)和毛根的性别鉴定。扩增的靶序列位于Y染色体DNA特异3.4kb重复序列中,扩增产物为460bp。检材用量为:新鲜血液0.5μl、血痕纱纤维1mm、毛根单个。20例保存4个月的血痕与2例保存6年半的血痕性别判定结果均正确,无性别记载的保存9～11年的3例血痕显现了清晰的460bpY特异DNA扩增带。15例保存20天的自然脱落毛根性别判定结果均正确。本法省略了检材处理中的酚-氯仿抽提DNA等纯化步骤,既简化了实验操作,又减少了检验过程中外源DNA的污染机会和样品DNA的损耗,使这一性别鉴定方法更符合法医学实践的需要。
The authors used primers Y_3, Y_4 and DNA polymerase chain reaction (PCR) for traceability of human blood (marks) and hairy root sex identification. The amplified target sequence was located on the Y chromosome DNA-specific 3.4kb repeats and the amplified product was 460bp. The amount of samples: fresh blood 0.5μl, bloodstained yarn fiber 1mm, hair root single. 20 cases of blood stained for 4 months and 2 cases of blood stained for 6 years and 6 years confirmed the results of the judgment. The 3 cases of blood streaks stored for 9 to 11 years without sex showed a clear 460bpY specific DNA amplification band. 15 cases of preserved 20 days of natural off hairy hair determination results are correct. This method omits the purification steps such as phenol-chloroform extraction DNA in the sample processing, which simplifies the experimental operation and reduces the chance of contamination of exogenous DNA and the loss of sample DNA during the inspection process to make this gender identification method more in line with The need of forensic practice.