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Cystatin C_2是从鸡孵清中提纯的巯基蛋白酶抑制肽。本研究观察Cystatin C_2对SHR主动脉平滑肌细胞(SMC)DNA合成的影响,并通过与内皮生长因子(EGF)、胰岛素,木瓜蛋白酶等作用的相互关系,探讨其作用机理。1×10~4SMC接种于96孔培养板,在含10%小牛血清的DMEM培养液中培养24小时后再在
Cystatin C_2 is a thiol protease inhibitor purified from chicken hatching. This study was designed to investigate the effect of Cystatin C_2 on the DNA synthesis of SMC aorta smooth muscle cells (SHRs) and to explore its mechanism through the interaction with Cytatin C 2 and EGF, insulin and papain. 1 × 10 ~ 4SMC were seeded in 96-well culture plates in DMEM medium containing 10% fetal bovine serum for 24 hours before