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以抗霜霉病的‘左山一’和易感病的‘双丰’山葡萄(Vitis amurensis Rupr.)为试材,离体叶片接种霜霉病菌后观察叶绿素荧光成像及参数变化。结果显示:叶绿素荧光成像系统可在接种霜霉病菌3 d时观察到病斑;霜霉病侵染叶片组织会显著改变叶绿素荧光参数;接种7 d时病斑与不接种对照相比,抗病品种‘左山一’Fv/Fm、ФPSⅡ、Y(NPQ)与Y(NO)的变化幅度(–73.42%、–100%、–49.64%、+3285.21%)均大于易感病品种‘双丰’的变化幅度(–22.98%、–28.17%、+5.62%、+26.18%),‘左山一’病斑周围组织的ФPSⅡ与r ETR高于对照,而‘双丰’低于对照。由此可见,‘左山一’能够较早形成枯斑,快速改变病斑处的荧光参数,并提高病斑周围组织的ФPSⅡ与r ETR值,从而增强对霜霉病的抗病性。因此,病斑周围组织的ФPSⅡ与r ETR可以作为初步筛选山葡萄抗霜霉病种质的指标。
Vitis amurensis Rupr. (Resistant to downy mildew) and Vitis amurensis Rupr. (Susceptible to disease) were used as materials. Chlorophyll fluorescence imaging and parameter changes were observed after in vitro leaf inoculation with downy mildew. The results showed that the chlorophyll fluorescence parameters could be significantly changed by downy mildew inoculation on the 3rd day after inoculation of downy mildew inoculation with the chlorophyll fluorescence imaging system. Compared with the non-inoculated control, The range of variation of Fv / Fm, ФPSⅡ, Y (NPQ) and Y (NO) was greater than that of susceptible variety ’Shuangfeng’ ’’ (-22.98%, -28.17%, + 5.62%, + 26.18%). The ФPSⅡand rTRR in the tissues around ZOAMANI were higher than those in the control, but the ’double peaks’ were lower than those in the control. Thus, ’Zusanmen-1’ can quickly form the dead spot, quickly change the fluorescence parameters at the lesion and improve the ФPS Ⅱ and r ETR values around the lesion, thereby enhancing the resistance to downy mildew. Therefore, ФPS ¢ ò and r-ETR around the lesion can be used as an index for primary screening of grapevine germplasm with resistance to downy mildew.