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本文介绍猪皮真皮胶原的分离和提纯。将胶原涂在F46膜上,培养人的表皮细胞获得成功。把培养5～13d的表皮细胞片自体移值,5例小面积成功。移植区活组织检查有新生的表皮屋。胶原可促进表皮细胞生长和连片,与对照组比较P<0.001。由于表皮片有胶原膜和F_(46)膜作底物,移植时从平皿中取出容易,不需要DispaseⅡ酶分离,反贴在切痂后2～5d或新鲜的肉芽创面。
This article describes the separation and purification of pigskin dermal collagen. The collagen coated on the F46 membrane, cultured human epidermal cells to be successful. The cultured 5 ~ 13d epidermal cell sheet self-displacement, 5 cases of small area success. Transplants biopsy has a new skin house. Collagen can promote epidermal cell growth and contiguous, compared with the control group P <0.001. As the epidermis with collagen film and F_ (46) film as a substrate, removed from the plate when transplanted easily, do not need Dispase Ⅱ enzyme separation, anti-posted in the scab 2 ~ 5d or fresh granulation wounds.