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目的 :研究胚胎脊髓 ,肋间神经 ,大网膜 ,椎旁肌移植到宿主脊髓后发育过程中形态变化。方法 :应用脊髓半切洞模型 ,移植后 1,2 ,4,12周 ,进行HE、Nissl、嗜银染色和电镜检查 ,观察移植物存活、分化及其与宿主之间的关系。结果 :胚胎脊髓移植到损伤的脊髓后 ,可在宿主脊髓内继续分化发育 ,与宿主脊髓形成部分纤维连接 ;肋间神经移植后 ,可能通过存活的雪旺氏细胞与宿主脊髓建立一定的结构联系 ;带蒂的大网膜移植后 ,可与宿主脊髓形成血管连接 ;带蒂的椎旁肌移植最终形成瘢痕组织。结论 :胚胎脊髓组织能较好地修复损伤的脊髓。
OBJECTIVE: To study the morphological changes of embryonic spinal cord, intercostal nerve, omentum and paravertebral muscle after their transplantation into the spinal cord of the host. Methods: The models of hemisection of spinal cord were established. At 1, 2, 4 and 12 weeks after transplantation, HE, Nissl, silver staining and electron microscopy were performed to observe the graft survival, differentiation and its relationship with the host. RESULTS: Embryonic spinal cord transplantation to the damaged spinal cord could continue to differentiate and develop in the spinal cord of the host and form part of the fiber connection with the spinal cord of the host. After the intercostal nerve transplantation, some structural connections may be established with the surviving Schwann cells and the spinal cord of the host ; Pedicled omental transplants, can form vascular connections with the host spinal cord; pedicle paravertebral muscle transplantation eventually the formation of scar tissue. Conclusion: Embryonic spinal cord tissue can repair damaged spinal cord well.