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丘脑底核(STN)深部脑刺激(DBS)已成为帕金森病的重要外科治疗手段,然而其确切的作用机理尚不明确。本研究采用微机电系统(MEMS)技术制备了一种16通道植入式微电极阵列(MEA),在MEA表面修饰了铂黑-还原氧化石墨烯-Nafion膜(Pt/RGO/Nafion)纳米材料,用于同步检测麻醉大鼠脑内纹状体神经元在STN电刺激前后多巴胺(DA)含量和动作电位(Spike)发放变化。STN-DBS结果表明,电刺激20 s后,DA含量开始升高,最高达1.72μmol/L,较高浓度状态保持约50 s后回落至正常水平。与此同时,检测到在DA上升阶段中间神经元Spike发放活动增强,在保持高于DA正常浓度水平阶段,中等多棘神经元(MSNs)放电频率增加。本研究制备的微电极阵列传感器能够实现脑内多巴胺和电生理的原位实时检测,有望成为神经信息检测的有力工具。
Subthalamic nucleus (STN) deep brain stimulation (DBS) has become an important surgical treatment of Parkinson’s disease, but its exact mechanism of action is not clear. In this study, a 16-channel implanted microelectrode array (MEA) was prepared by MEMS technology. Pt / RGO / Nafion nanocomposites were modified on the surface of MEA. To detect synchronously the changes of dopamine (DA) content and action potential (Spike) in striatum neurons in the brain of anesthetized rats before and after STN stimulation. The results of STN-DBS showed that after 20 s of electrical stimulation, the content of DA began to increase up to 1.72 μmol / L, and returned to the normal level after about 50 s at the higher concentration. In the meantime, Spike release activity was detected during the ascent phase of DA, and the discharge frequency of moderate multi-spine neurons (MSNs) increased during the period of keeping the normal concentration level above DA. The microelectrode array sensor prepared in this study can realize in-situ real-time detection of dopamine and electrophysiology in the brain and is expected to become a powerful tool for nerve information detection.