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本文对32例扁桃体炎患者切除的扁桃体标本应用双染色流式细胞仪(FCM)测定淋巴细胞亚群及其活性。患者分两组,21岁以上16例(成人组),15岁以下16例(儿童组)。肥大型扁桃体炎14例,复发型扁桃体炎18例。淋巴细胞与用异硫氰基荧光素(FITC)或藻红蛋白(PE)标记的相应特异单克喹抗体结合后进行 FCM 测定。结果发现扁桃体组织中辅助 T 细胞(CD_4~+Leu8~-)较外周血显著增高(P<0.005),而诱导 T 细胞(CD_4~+Leu8~+)、抑制 T 细胞(CD8~+CD11~+)及细胞毒性 T 细胞(CD8~+CD11~-)较外周血少(P<0.005),尤其抑制细胞在扁桃体组织中非常少见。成人患者扁桃体组织中四种 T 细胞所占比例均不同程度地较儿童患者高。肥大型组的细胞毒性 T 细胞所占比例明显高于复发型组(P<0.05),而复发型组的诱导 T 细胞和抑制 T 细胞所占比例又明显高于肥大型组(P<0.005)。外周血活性 CD4~+或 CD8~+细胞不到总淋巴细胞的1%,
In this study, 32 patients with tonsillitis resected tonsil specimens using double staining flow cytometry (FCM) determination of lymphocyte subsets and their activity. The patients were divided into two groups, 16 cases over 21 years old (adult group) and 16 cases under 15 years old (child group). Fourteen cases of hypertrophic tonsillitis, recurrent tonsillitis in 18 cases. FCM assays were performed after lymphocytes bound to the corresponding specific monoclonal monoclonal antibodies labeled with isothiocyanofluorescein (FITC) or phycoerythrin (PE). The results showed that T cells (CD_4 + Leu8 ~) in tonsil tissues were significantly higher than those in peripheral blood (P <0.005), but T cells (CD8 + CD11 + ) And cytotoxic T cells (CD8 ~ + CD11 ~ -) than the peripheral blood less (P <0.005), in particular inhibition of cells in tonsil tissue is very rare. The proportion of all four T cells in tonsil tissues in adult patients was significantly higher than that in pediatric patients. The proportion of cytotoxic T cells in the hypertrophic group was significantly higher than that in the recurrent group (P <0.05), while the proportion of induced T cells and T cells in the recurrent group was significantly higher than that in the hypertrophic group (P <0.005) . Peripheral blood CD4 ~ + or CD8 ~ + cells less than 1% of total lymphocytes,