Study on Seed Germination Conditions and in vitro Tissue Culture of Lilium formolongi

• 作 者: Bo WANG Yongping ZHU Xiaojie QIN Qiongjie LI Xiaoqiao WANG Fengmei HE
• 机 构: College of Horticulture and Landscape,Yunnan Agricultural University,
• 出 处: Agricultural Biotechnology
• 发表日期: 2012年03期
• 关键词: Lilium illumination germination incubation inductive callus inducing facilitate

To facilitate the rapid production of lily(Lilium formolongi),conditions favoring the germination of dormant lily seeds and in vitro regeneration from tissue culture were studied,using the germinating plumule as the explants.The optimal inducing conditions for dormant lily dry seeds to germinate were low temperature(10 ℃) treatment for one hour followed by incubating at 18 ℃ with light illumination.After 8 days of incubation/induction,over 90% of the seeds were germinated.MS+6-BA 1.0 mg/L +NAA 0.5 mg/L was the most efficient medium formula in lily callus induction.MS+6-BA 2.0 mg/L +NAA 0.5 mg/L was the optimal medium for calli growth.1/2MS+NAA 1.0 mg/L was the medium gave the highest root inductive rate.

To facilitate the rapid production of lily (Lilium formolongi), conditions favoring the germination of dormant lily seeds and in vitro regeneration from tissue culture were using, the germinating plumule as the explants. The optimal inducing conditions for dormant lily dry seeds to germinate were over 90% of the seeds were germinated. MS + 6-BA 1.0 mg / L + NAA 0.5 mg at low temperature (10 ° C) treatment for one hour followed by incubating at 18 ° C with light illumination / L was the most efficient medium formula in lily callus induction. MS + 6-BA 2.0 mg / L + NAA 0.5 mg / L was the optimal medium for calli growth.1 / 2MS + NAA 1.0 mg / L was the medium gave the highest root inductive rate.