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目的:研究血水草生物碱(Eomecon chionantha Hance alkaloids,ECA)对钉螺足跖平滑肌收缩活动的影响及与毒蕈碱型乙酰胆碱受体(muscarinic acetylcholine receptors,mAChR)的关系,探讨其减低钉螺附壁上爬、增强灭螺效果的作用机制。方法:采用离体钉螺足跖平滑肌实验方法,观察ECA对钉螺足跖平滑肌收缩活动的影响。结果:5.0、10.0、20.0 mg/L ECA均可增加钉螺足跖平滑肌张力、减小收缩幅度、加快收缩频率;2×10-6mol/L阿托品可明显降低由ECA所至的钉螺足跖平滑肌的张力,而对其所至的收缩幅度及收缩频率影响不明显;6×10-6mol/L阿托品则可显著地拮抗ECA引起的钉螺足跖平滑肌兴奋作用,使钉螺足跖平滑肌的张力及收缩幅度均明显降低,但对其所至收缩频率影响不明显。结论:ECA能兴奋钉螺足跖平滑肌;ECA引起的钉螺足跖平滑肌兴奋作用部分由mAChR介导,其作用机制可能与激活受体依赖性钙通道(receptor operated calcium channel,ROCC)有关。
Objective: To investigate the effect of ECA on contractile activity of plantar flexor muscle of Scylla vulgaris and its relationship with muscarinic acetylcholine receptors (mAChR) Climb, enhance the mechanism of the effect of snail control. Methods: The effects of ECA on contractile activity of plantar flexor spinae flexorhiza were observed by the method of exfoliating the plantar smooth muscle of snail. Results: 5.0, 10.0 and 20.0 mg / L ECA could increase the plantar flexion and contractility, and accelerate the contractile frequency. Atropine at 2 × 10-6 mol / L could significantly reduce the contralateral plantar flexion induced by ECA Tension, and its contraction rate and contraction frequency is not obvious; 6 × 10-6mol / L atropine can significantly antagonize ECA-induced snail plantar plantar smooth muscle excitability, so that snail plantar plantar muscle tension and contraction amplitude Were significantly lower, but its impact on the contraction frequency is not obvious. CONCLUSION: ECA can excite the plantar smooth muscle of the snail. The excitatory effect of ECA-induced plantar smooth muscle of the snail is partly mediated by mAChR, which may be related to the activation of receptor-operated calcium channel (ROCC).