论文部分内容阅读
目的本研究旨在探讨华北地区Fas-670和FasL-844基因多态性是否与胃癌易感性有关联。方法提取234例胃癌患者及321例健康献血者外周血基因组DNA,用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)确定Fas-670和FasL-844基因型。结果 Fas-670和FasL-844基因型在病例组和对照组中的分布差异无统计学意义(P>0.05)。Logistic回归分析表明,Fas-670和FasL-844基因多态性与胃癌发生风险无相关性。分层分析显示,在吸烟人群中,相对于携带Fas-670AA基因型而言,携带Fas-670GA基因型患胃癌的风险高(OR=2.438,95%CI 1.067～5.569)。结论在华北地区,FasL-844基因多态性与胃癌发生风险无相关性,而Fas-670GA基因型吸烟者有较高的患胃癌风险。
Objective This study aimed to investigate whether the gene polymorphisms of Fas-670 and FasL-844 in North China are associated with gastric cancer susceptibility. Methods Genomic DNA was extracted from 234 gastric cancer patients and 321 healthy blood donors. The genotypes of Fas-670 and FasL-844 were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There were no significant differences in the distribution of Fas-670 and FasL-844 genotypes between cases and controls (P> 0.05). Logistic regression analysis showed that there was no correlation between the polymorphisms of Fas-670 and FasL-844 and the risk of gastric cancer. Hierarchical analysis showed that there was a high risk of gastric cancer with the Fas-670GA genotype (OR = 2.438, 95% CI 1.067-5.569) in smokers relative to the Fas-670AA genotype. Conclusion There is no correlation between FasL-844 polymorphism and the risk of gastric cancer in North China, while Fas-670GA genotype smokers have a higher risk of gastric cancer.