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目的:探索天麻川芎(TC)对自发高血压大鼠炎症因子的影响。方法:自发高血压大鼠(SHR)分为SHR组、卡托普利组(90 mg·kg~(-1)·d~(-1))、TC大剂量组(18.4 g·kg~(-1)·d~(-1))和小剂量组(9.2 g·kg~(-1)·d~(-1)),WKY为正常对照。灌胃给药8周,给药前及给药后每两周测定血压一次。给药结束后,ELISA法测定血浆CRP水平;RT-PCR法测定心肌及主动脉中的TNF-α、IL-6、IL-1βmRNA表达水平。结果:CT小、大剂量组对SHR均无显著降底SHR收缩压作用,但可均可显著降低SHR组的血浆CRP水平(P<0.05)及主动脉及心肌中的TNF-α、IL-6、IL-1βmRNA的表达水平,P<0.05。结论:天麻川芎可降低自发高血压大鼠血浆CRP及主动脉、心肌炎症因子TNF-α、IL-6、IL-1βmRNA的表达水平。
Objective: To explore the effects of Tianma Chuanxiong (TC) on inflammatory cytokines in spontaneously hypertensive rats. SHR group, captopril group (90 mg · kg -1 · d -1), high dose of TC group (18.4 g · kg ~ (-1)), spontaneous hypertensive rats -1) · d -1 and low dose group (9.2 g · kg -1 · d -1), WKY was normal control. Gavage for 8 weeks, before administration and after administration of blood pressure once every two weeks. Serum CRP levels were measured by ELISA and mRNA expression of TNF-α, IL-6 and IL-1β in myocardium and aorta were detected by RT-PCR. Results: There was no significant difference in the SHR systolic pressure between the SHR group and the small and high dose CT groups, but both of them could significantly decrease the plasma CRP level (P <0.05) and the levels of TNF-α and IL- 6, IL-1βmRNA expression level, P <0.05. Conclusion: Tianma Chuanxiong can reduce the levels of plasma CRP, aortic and myocardial inflammatory cytokines TNF-α, IL-6 and IL-1βmRNA in spontaneously hypertensive rats.