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为了实现对液态环境中微球三维位置的快速精密测量,尤其是轴向的测量分辨力和速度,基于离焦成像测量理论,结合并改进象限插值法和矢径投影算法,提出了一种新的方法,实现了对微球三维方向1nm测量分辨力的跟踪测量。该方法测量效率高,对单个粒子轴向位置的测量速率最快达到每秒上百帧甚至更高。进一步讨论了横向位置测量误差对轴向测量分辨力的影响,分析了轴向方向大范围的跟踪测量分辨力。通过和相同实验条件下的互相关方法对比,说明该方法在实际测量中具有可行性,在实现相同轴向测量分辨力的情况下测量效率更高。
In order to realize fast and precise measurement of three-dimensional position of microsphere in liquid environment, especially axial resolving power and resolution, based on the theory of defocus imaging measurement, combined with quadrant interpolation and radial projection algorithm, a new Method, to achieve the microsphere three-dimensional direction 1nm measurement resolution of the tracking measurement. The method has the advantages of high measurement efficiency and the fastest measurement rate of the axial position of individual particles reaches hundreds of frames per second or even higher. The influence of lateral position measurement error on axial measurement resolution is further discussed, and a large range of tracking measurement resolution in axial direction is analyzed. The comparison with the cross-correlation method under the same experimental conditions shows that this method is feasible in practical measurement, and it is more efficient in measuring the same axial resolution.