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[目的]从分子水平上研究内蒙古草原主要蝗虫种类的遗传进化与系统发育关系。[方法]采用随机扩增多态性DNA(RAPD)技术对蝗总科4科6属8种蝗虫的80个个体进行扩增,对基因组DNA进行多态性比较。[结果]7条引物共扩增出64条特异性片断,分子量大小为300～2000bp。8种蝗虫间遗传距离在0.2282～0.5896。带型显示,科内属间及属内种间多态性普遍存在。根据Nei's遗传距离对结果进行UPG-MA法聚类分析,结果表明,属内的种各自先聚为一类,其次是科内的种聚为一类。[结论]该研究可为内蒙古草原主要蝗虫的系统发育及演化研究提供分子生物学依据。
[Objective] The research aimed to study the genetic evolution and phylogenetic relationship of major grasshopper species in Inner Mongolia grassland at the molecular level. [Method] 80 individuals of 8 locusts, 6 genera, 4 genera, 4 families, of Acridoidea were amplified by random amplified polymorphic DNA (RAPD) technique, and the polymorphism of genomic DNA was compared. [Result] A total of 64 specific fragments were amplified with 7 primers and the molecular weight was 300-2000bp. The genetic distance among 8 locusts ranged from 0.2282 to 0.5896. Belt-type display, within the genus and genus interspecific polymorphism is widespread. According to Nei's genetic distance, UPG-MA method was used to cluster the results. The results showed that the species in the genus were clustered into one category, followed by the species within the family. [Conclusion] This study could provide molecular biological basis for the phylogeny and evolution of major locusts in Inner Mongolia grassland.