论文部分内容阅读
目的:从凋亡之异常角度探讨儿童髓母细胞发生、发展及预后的生物学机制。方法:以84例获随访的儿童髓母细胞瘤组织(按术后生存期3、5、10年分为A、B、C三组)为研究对象,用原位杂交及免疫组化染色法分别检测Bcl-2mRNA和增殖细胞核抗原(PCNA)的表达,并用31末端标记法做原位细胞凋亡检测。结果:72例(85.7％)表达Bcl-2mRNA,其阳性率为A>B>C;PCNA阳性肿瘤组织细胞密度与肿瘤细胞Bcl-2mRNA表达水平增加,呈正相关,而凋亡肿瘤细胞密度与肿瘤细胞Bcl-2mRNA表达水平呈负相关。结论:儿童髓母细胞瘤中Bcl-2基因高表达可抑制其凋亡,细胞增殖与凋亡失衡可能是儿童髓母细胞瘤发生发展的重要环节。
Objective: To explore the biological mechanism of the occurrence, development and prognosis of children’s medullary cells from the perspective of apoptosis. Methods: Totally 84 children with medulloblastoma (follow-up survival time of 3, 5, 10 years were divided into A, B, C three groups) as the research object, using in situ hybridization and immunohistochemistry The expression of Bcl-2 mRNA and proliferating cell nuclear antigen (PCNA) were detected respectively, and the apoptosis in situ was detected by 31 end-labeling method. Results: 72 cases (85.7%) expressed Bcl-2 mRNA, the positive rate was A> B> C. The cell density of PCNA positive cells was positively correlated with the increase of Bcl-2 mRNA expression in tumor cells, The expression of Bcl-2 mRNA was negatively correlated. CONCLUSION: High expression of Bcl-2 gene in childhood medulloblastoma can inhibit its apoptosis. The imbalance of cell proliferation and apoptosis may be an important link in the development of childhood medulloblastoma.