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目的通过观察妊娠期肝内胆汁淤积症(ICP)与正常妊娠孕妇的胎盘来源的单核-巨噬细胞中受体O型蛋白酪氨酸磷酸酶(Protein tyrosine phosphatase receptor-type O,PTPRO)的表达情况、细胞因子的分泌及胞内信号分子NF-κB/P65活化水平的变化,探讨PTPRO在妊娠胎盘免疫微环境的平衡维持中的作用。方法选取3组孕妇:正常妊娠(26例)、ICP轻型(24例)及ICP重型孕妇(24例),分离并由密度-梯度离心法获得其胎盘来源的单核-巨噬细胞,ELISA法检测细胞培养上清液中TNF-α、IFN-γ、IL-6的水平;Real-time PCR及Western blot法分别检测PTPRO mRNA、蛋白水平的表达以及NF-κB/P65的磷酸化改变。结果与正常妊娠者相比,ICP孕妇胎盘来源的单核-巨噬细胞高分泌TNF-α、IFN-γ、IL-6(P均<0.01),NF-κB/P65的磷酸化增多,ICP轻型及重型孕妇胎盘来源的单核-巨噬细胞细胞因子PTPRO的表达上调。结论 ICP孕妇胎盘组织内炎性因子浸润,可能与PTPRO、NF-κB/P65的相互调控有关。
Objective To investigate the effect of intrahepatic cholestasis of pregnancy (ICP) on the expression of placental-derived monocyte-macrophage receptor protein tyrosine phosphatase (PTPRO) in normal pregnant women The secretion of cytokines and the activation of intracellular signaling molecule NF-κB / P65 in order to explore the role of PTPRO in the balance maintenance of placental immune microenvironment. Methods Three groups of pregnant women were selected: normal pregnancy (26 cases), ICP light type (24 cases) and ICP heavy pregnant women (24 cases). Mononuclear macrophages derived from placenta were isolated by density gradient centrifugation. The levels of TNF-α, IFN-γ and IL-6 in cell culture supernatants were detected. The expression of PTPRO mRNA and protein and the phosphorylation of NF-κB / P65 were detected by Real-time PCR and Western blot respectively. Results Compared with normal pregnant women, placenta-derived monocyte-macrophages secreted high levels of TNF-α, IFN-γ and IL-6 in ICP pregnant women (all P <0.01), phosphorylation of NF-κB / Placenta-derived monocyte-macrophage cytokine PTPRO is up-regulated in both light and heavy pregnant women. Conclusion The infiltration of inflammatory factors in placenta of ICP pregnant women may be related to the mutual regulation of PTPRO and NF-κB / P65.