论文部分内容阅读
目的:探讨了抗包虫药物对细粒棘球蚴囊壁谷胱甘肽硫转移酶(GST)和超氧化物歧化酶(SOD)的影响,用感染细粒棘球蚴原头节达10—12个月的小鼠作试验.方法:GST用氯硝基苯与还原型谷胱甘肽结合法测定.SOD用改进的邻苯三酚自氧化法测定.结果:囊壁GST和SOD的活力各为12.3±4.3—13.5±4.8 μmol min~(-1)/mg蛋白质,和4.4±2.9—6.1±1.4 U min~(-1)/mg蛋白质.感染鼠用甲苯达唑(Meb)25 mg kg~(-1)d~(-1)治疗14 d时,瘪囊与充盈囊壁的GST活力各被抑制30％和26.8％,而SOD活力则升高105％—163％.阿苯达唑300 mg kg~(-1)d~(-1)和吡喹酮500mgkg~(-1)d~(-1),连给14 d,对上述2种酶无明显影响.结论:Meb抑制囊壁的GST可能损害囊防御系统.
Objective: To investigate the effects of anti-echinococcosis drugs on glutathione S-transferase (GST) and superoxide dismutase (SOD) in the cystic wall of Echinococcus granulosus, 12 months mice.Methods: GST was determined by the combination of chloronitrobenzene and reduced glutathione.ODE was measured by modified pyrogallol autoxidation.Results: The activity of GST and SOD (12.3 ± 4.3-13.5 ± 4.8 μmol min -1 / mg protein, and 4.4 ± 2.9-6.1 ± 1.4 U min -1 mg protein, respectively.) Infection with rat mebendazole (Meb) 25 mg At 14 days after treatment with kg ~ (-1) d ~ (-1), the GST activities of the deflated cysts and the inflated cyst wall were inhibited by 30% and 26.8%, while the SOD activity increased by 105% -163% There was no significant effect on the above two enzymes when azole 300 mg kg -1 d -1 and praziquantel 500 mg kg -1 d -1 for 14 d.Conclusion: GST wall may damage the capsule defense system.