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The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters(PM_(2.5)) on rat alveolar macrophages(AMs) in vitro.Morphologies and element concentrations of chalk particles were analyzed using quantitative energydispersive electron probe X-ray microanalysis(ED-EPMA).The oxidative response of AMs exposed to chalk PM_(2.5) was measured by luminol-dependent chemiluminescence(CL).The results showed that(1)Chalk dust was mainly composed of gypsum(CaSO_4),calcite(CaCO_3)/dolomite(CaMg(CO_3)_2),and organic adhesives;(2) Fine chalk particles induced the AM production of CL,which was inhibited by about 90%by diphenyleneiodonium chloride(DPI).Based on these results,we showed that cytotoxicity of chalk PM_(2.5) may be related to the reactive oxygen species(ROS) generation.
The aim of this study was to investigate the adverse effects of fine chalk particle matters (PM_ (2.5)) on rat alveolar macrophages (AMs) in vitro. Morphologies and element concentrations of chalk particles were analyzed using Quantitative energydispersive electron probe X-ray microanalysis (ED-EPMA). The oxidative response of AMs exposed to chalk PM_ (2.5) was measured by luminol-dependent chemiluminescence (CL). The results showed that gypsum (CaSO_4), calcite (CaCO_3) / dolomite (CaMg (CO_3) _2), and organic adhesives; (2) Fine chalk particles induced the AM production of CL, which was inhibited by about 90% by diphenyleneiodonium chloride (DPI). Based on these results, we showed that cytotoxicity of chalk PM_ (2.5) may be related to the reactive oxygen species (ROS) generation.