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目的:探索在位子宫内膜间质细胞体外分离、纯化、培养的方法,从而建立研究子宫内膜异位症(EMs)的体外细胞模型。方法:选择因子宫肌瘤或子宫腺肌症行子宫(次)全切除术的病例38例,术中取其子宫内膜,通过酶解、过滤、贴壁纯化等技术,分离培养子宫内膜间质细胞并进行传代。结果:36例标本获得成功,分离纯化得到的子宫内膜间质细胞经角蛋白、波形蛋白染色证实纯度达95%以上,活性较强,可以有限传代,传代培养的细胞形态特征与原代细胞一致。结论:该方法可以高效简便地分离在位子宫内膜间质细胞,得到的间质细胞产量及纯度高,可以作为EMs的体外细胞模型之一。
Objective: To explore the method of in vitro isolation, purification and culture of endometrial stromal cells in vitro and to establish an in vitro cell model for the study of endometriosis (EMs). Methods: Thirty-eight cases of uterine (sub) total resection due to uterine fibroids or adenomyosis were selected. The uterus was taken from the endometrium during operation, and the endometrium was isolated and cultured by enzymolysis, filtration and adherent purification Interstitial cells and passage. RESULTS: Thirty-six specimens were successfully obtained. The stromal cells isolated and purified from uterine endometrium were proved to be over 95% pure by keratin and vimentin staining. Their activity was high and they could be passaged for a limited time. The morphological characteristics of cells in subculture were similar to that of primary cells Consistent. CONCLUSION: This method can efficiently and conveniently isolate the endometrial stromal cells in the endometrium. The obtained stromal cells have high yield and purity and can be used as one of the in vitro cell models of EMs.