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目的 寻找新的高效杀伤活性的细胞 ,探讨脐血单个核细胞作为LAK细胞前体细胞的可行性和意义。方法 分离的脐血单个核细胞 ,分别用IL -2和IL -2 +IFNα诱导LAK细胞 ,并于培养后d 2 ,4,7,11,14分别测其细胞扩增数量和对K5 62细胞的杀伤活性。结果 于培养后d 11时 ,脐血单个核细胞的扩增倍数最高 ,两组分别为培养前的 2 0倍和 2 5倍 ,其对K5 62的杀伤活性分别为对照组培养前的 2 7倍和 3 5倍。结论 ①脐血单个核细胞是良好的LAK细胞的前体细胞 ;②其细胞数量和杀伤能力在培养的d 11达高峰 ;③IL -2 +IFNα组的细胞数量和杀伤活性优于单用IL -2组
OBJECTIVE: To search for new highly active and lethal cells and to explore the feasibility and significance of umbilical cord blood mononuclear cells as precursor cells of LAK cells. Methods The human umbilical cord blood mononuclear cells were isolated and induced with IL-2 and IL-2 + IFNα, respectively. The number of LAK cells was measured at day 2, 4, 7, The killing activity. Results At d 11, the multiplication of cord blood mononuclear cells was the highest, 20 and 25 times higher than that before culture, and the killing activity of K562 was 27 Times and 3 times. Conclusions ① Umbilical cord blood mononuclear cells are the precursor of LAK cells. ② The number and cytotoxicity of LAK cells reach the peak on d 11 of culture. ③ The cell number and cytotoxicity of IL - 2 + 2 teams