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本文利用一种自制的真菌细胞壁溶解酶——溶壁酶(Lywallzyme),通过酶解试验比较了菌龄、渗透压稳定剂、酶解温度及酶解时间等对凤尾菇菌丝原生质体分离的影响。通过原生质体的再生试验,系统地比较了菌龄、渗透压稳定剂和酶解时间及再生培养基对凤尾菇菌丝原生质体再生频率的影响,从而找到凤尾菇茵丝原生质体分离和再生的较适条件。在适宜的酶解条件下可分离到大量有活力的原生质体,其产量最高可达3×10~7个/me以上。本试验中,原生质体的再生频率最高为4.98％。同时,本文还对凤尾菇菌丝原生质体再生过程的形态变化作了适当描述。
In this paper, a kind of self-made fungal cell wall lytic enzyme (Lywallzyme) was used to separate mycelia of Pleurotus ferulae by means of enzymolysis test, such as bacterial age, osmotic pressure stabilizing agent, enzymolysis temperature and enzymolysis time Impact. Through protoplast regeneration experiments, we systematically compared the effects of bacterial age, osmotic pressure stabilizing agent, enzymolysis time and regeneration medium on the regeneration frequency of Pleurotus ostreatus mycelium protoplasts to find the protoplast isolation and Regeneration of the more appropriate conditions. A large number of viable protoplasts can be isolated under the appropriate conditions of enzymolysis and the yield can reach up to 3 × 10 ~ 7 / me. In this experiment, the highest frequency of protoplast regeneration was 4.98%. At the same time, this article also describes the morphological changes of protoplast regeneration of Pleurotus ostreatus.