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众所周知,动物和人类高血压性心肌肥厚时,心肌重量与动脉血压之间并无严格的对应关系;即除血压外,还存在某些调节心肌肥厚发生和发展的因子.作者从自发性高血压鼠(SHR)心肌匀浆中分离并提纯获得一种部分纯化的因子,另从实验性肾动脉狭窄性高血压Wistar鼠(RHR)心脏分离制备心肌细胞悬液.RHR心肌细胞悬液内加入SHR心肌因子并在体外孵育后,经测定发现其蛋白质合成速率高于未加入SHR心肌因子的对照悬液.这表现为心肌细胞肌球蛋白内掺入的~3H-亮氨酸较多(1285±29对975±35dpmg,p<0.05)、亮氨酸tRNA比活性较强(89±3.5对56±4.5dpm/pmol,p<0.05)和蛋白质合成指数较高(7.0±2.0对4.02
As we all know, animal and human hypertensive cardiac hypertrophy, myocardial weight and arterial blood pressure and there is no strict correspondence between; that is, in addition to blood pressure, there are some factors that regulate the occurrence and development of cardiac hypertrophy.From the spontaneous hypertension (SHR) were isolated and purified to obtain a partially purified factor, and the other was isolated from the heart of experimental renal hypertensive rat Wistar rat (RHR) to prepare myocardial cell suspension.RHR cardiomyocyte suspension was added SHR Myocardial factors, after in vitro incubation, were found to have a higher rate of protein synthesis than the control suspension without the addition of SHR, which is manifested by the increased incorporation of ~ 3H-leucine into the myosin of cardiomyocytes (1285 ± 29 vs. 975 ± 35 dpmg, p <0.05), leucine tRNA was more specific (89 ± 3.5 vs 56 ± 4.5 dpm / pmol, p <0.05) and had a higher protein synthesis index (7.0 ± 2.0 vs. 4.02