复发性外阴尖锐湿疣HPV的检测及型别分析

• 作 者: 林霖霖 陈云琴 李智铭 张学奇 徐云升 胡燕 郑飞云 蔡剑峰 吴慧珍 林孝华 张洪勤
• 机 构: 浙江省温州医学院附属第一医院,浙江省温州医学院附属第一医院,浙江省温州医学院附属第一医院,浙江省温州医学院附属第一医院,浙江省温州医学院附属第一医院,浙江省温州医学院附属第一医院,浙江省温州医学院附属
• 出 处: 中国麻风皮肤病杂志
• 发表日期: 2007年05期
• 关键词: 复发性 尖锐湿疣 聚合酶链反应

采用聚合酶链反应(PCR)方法结合核酸斑点杂交技术,对45例外阴复发性尖锐湿疣(RCA)进行HPV DNA的检测及分型。结果45例RCA中HPV DNA阳性40例,HPV检出率为88.8%。健康对照组各型均阴性,差异有极显著性(P<0.001)。40例RCA中低危型HPV6、11分别为27.5%、22.5%;高危型HPV16、18未检出;高危型HPV33为5%。其中HPV6、11、16、18、33多重型别感染的病程大于7个月以上较单一型感染的检出率明显升高(85.7%vs14.2%,P<0.001)。RCA多重型别感染与对照初发CA多重型别感染检出率比较差异有显著性(45%vs11.1%,P<0.05)。温州地区复发性尖锐湿疣以多重型别混合感染为主,且与临床病程迁延,病情复发存在着一定的相关性。

Polymerase chain reaction (PCR) and nucleic acid dot blot were used to detect and classify HPV DNA in 45 cases of vulvar recurrent condyloma acuminatum (RCA). Results Of the 45 RCA cases, 40 cases were positive for HPV DNA, the detection rate of HPV was 88.8%. All types of healthy control group were negative, the difference was significant (P <0.001). In 40 cases of RCA, the low-risk HPV6 and 11 were 27.5% and 22.5% respectively; the high-risk HPV16 and 18 were not detected; the high-risk HPV33 was 5%. Among them, the detection rate of multiple genotypes of HPV6, 11, 16, 18 and 33 was higher than that of single genotypes more than 7 months (85.7% vs14.2%, P <0.001). There was a significant difference in the detection rate of multiple genotypes of RCA among multiple genotypes and controls (45% vs 11.1%, P <0.05). Wenzhou, recurrent genital warts with multiple types of mixed infections, and with the clinical course of the disease recurrence there is a certain correlation.