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目的对多种分子分型方法在单核细胞增生李斯特氏菌(Listeria monocytogenes,Lm)分型中的应用进行分析和比较,确定适合于不同实验室的分型检测方法,并进一步掌握食源性Lm的病原特征。方法采用分子血清分型、二元基因分型与多位点序列分型方法对分离自食品的69株Lm进行分型检测,并对结果进行分析。结果 69株Lm食品分离株中,1/2a型菌株有29株,1/2b型有19株,1/2c型有7株,4b型有14株。二元基因分型法将69株Lm食品分离株分为19个二元基因型(Binary type,BT型),DI值为91.60%;主要的BT型为BT146、BT254、BT222、BT82、BT159和BT190。多位点序列分型法将69株Lm食品分离株分为16个序列型(Sequencing type,ST型),DI值为89.98%;主要的ST型为ST8、ST87、ST2、ST1、ST3和ST9。结论分子血清学方法和二元基因分型方法具有操作简便、检测结果易于分析、较快提供检测结果的优点,可作为实验室的一线技术用以对李斯特氏菌病暴发的发现和确认。
Objective To analyze and compare the application of multiple molecular typing methods in the typing of Listeria monocytogenes (Lm), and to determine the suitable typing methods for different laboratories and to further understand the food source Pathogenic features of sexual Lm. Methods The molecular typing, binary genotyping and multilocus sequence typing were used to detect 69 strains of Lm isolated from food, and the results were analyzed. Results Among the 69 Lm food isolates, 29 strains were type 1 / 2a, 19 strains were type 1 / 2b, 7 were type 1 / 2c and 14 were type 4b. Ninety-two Lm strains were divided into 19 binary genotypes (Binary type, BT type) with DI value of 91.60% by binary genotyping. The main BT genotypes were BT146, BT254, BT222, BT82, BT159 and BT190. Sixty-nine Lm food isolates were divided into 16 sequences (ST type) with DI value of 89.98% by multisite sequence typing. The main ST types were ST8, ST87, ST2, ST1, ST3 and ST9 . Conclusion The molecular serological method and binary genotyping method have the advantages of simple operation, easy analysis of the test results and quick detection results, which can be used as a laboratory first-line technology for the discovery and confirmation of the listeriosis outbreak.