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描述一种叶组织内昆虫卵和幼虫染色技术。叶片先用改良 Carnoy 氏固定液(乙醇,氯仿,冰醋酸体积比6:4:3)处理除去叶绿素,然后用透明—染色液(1%酸性品红水溶液和透明液(20g 苯酚,5g 水合氯醛和100ml 乳酸)之比为1:10)染色卵。用0.1%孔雀绿乳酸液复染。卵被染成深红色,植物组织被染成绿色。该方法对菜豆蛇潜蝇Ophiomyia phaseoli(Tryon)、Dphiomyia sp.、叶蝉 Empoasca kerri Pruthi 和 Amrasca
Describe a technique for insect egg and larval staining in leaf tissue. The leaves were first treated with modified Carnoy’s fixative (ethanol, chloroform, glacial acetic acid in a volume ratio of 6: 4: 3) to remove chlorophyll and then treated with clear-staining solution (1% acidic magenta aqueous solution and clear solution (20 g phenol, Aldehyde and 100 ml of lactic acid) 1:10). Use 0.1% malachite green lactic acid solution stained. Eggs are dyed dark red and plant tissue is dyed green. The method for Ophiomyia phaseoli (Tryon), Dphiomyia sp., Leafhoppers Empoasca kerri Pruthi and Amrasca