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以豫麦13为父本,偃展1号为母本配制杂交组合构建分离群体,对豫麦13进行抗条锈病遗传分析,并采用SSR标记技术对豫麦13中抗条锈基因进行分子定位。豫麦13和偃展1号组合的F5代材料接种条中31号,其抗感株系分离比率为2.43∶1,接近3∶1的理论比值,表明豫麦13对条中31号的抗性由2对基因控制;在所用的290对SSR引物中,2B染色体上有Xgwm501、Xgwm120、Xgwm429、Xgwm374、Xwmc441、Xwmc360等6个标记与抗病基因连锁,在3B染色体上有Xwmc 3和Xgwm131等2个标记与抗病基因连锁,表明豫麦13中的抗条锈基因位于2B和3B染色体上。与已知的定位于2B和3B上的抗条锈基因进行比较分析,确定豫麦13中的抗条锈基因为不同于已知抗病基因的未知基因。
Yumai 13 was used as male parent and Yanzhan 1 as female parent to construct segregating population. The genetic analysis of resistance to stripe rust in Yumai 13 was carried out. The molecular marker of the stripe rust resistance gene in Yumai 13 was determined by SSR marker . The combination of Yumai 13 and Yanzhan 1 in F5 generation inoculation No. 31, the anti-susceptible strain separation ratio of 2.43: 1, close to the theoretical ratio of 3: 1, indicating Yumai 13 on the 31st Sex was controlled by 2 pairs of genes. Among the 290 pairs of SSR primers used, 6 markers, Xgwm501, Xgwm120, Xgwm429, Xgwm374, Xwmc441 and Xwmc360, were linked to the resistance gene on chromosome 2B, with Xwmc 3 and Xgwm131 on chromosome 3B Two markers were linked to the disease resistance genes, indicating that the stripe rust resistance genes in Yumai 13 are located on chromosomes 2B and 3B. Compared with the known anti-stripe rust genes located on 2B and 3B, the anti-stripe rust gene in Yumai 13 was determined to be an unknown gene different from the known resistance genes.