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在当阳市烟集乡卫生院的配合下,对当地有咳嗽病史的93例农民进行了“农民肺”的调查,应用热吸水链霉菌(H9—4株)、普通商温放线菌(101株)和干草小多孢菌(150B株)制备的三种抗原进行了血清学检查。间接血凝试验25人的阳性,阳性率为26.88％,经4个月后,对血凝阳性患者进行复查,并同时作双向琼脂扩散和SDS—PAGE免疫转印试验,结果基本一致。结合临床资料,证明烟集乡农民确有“农民肺”患者。此外,经病人痰液(7份)和堆草(3份)作病原菌分离培养,霉菌占优势,为今后开展普查,提供增加与之相应的霉菌菌株抗原的依据。
With the help of Dangyang Tobacco Township Hospital, 93 peasants who had a history of cough were investigated for “farmer lungs” by using Streptomyces hygroscopicus (H9-4 strain), common commercial temperature actinomycetes 101 strains) and D. multocida (150B strain) were tested for serology. Indirect hemagglutination test of 25 people were positive, the positive rate was 26.88%. After 4 months, patients with hemagglutination positive were reviewed, and two-way agar diffusion and SDS-PAGE immunoblotting tests were performed at the same time. The results were basically consistent. Combined with clinical data to prove that smoke farmers farmers really “farmer lung” patients. In addition, the patient sputum (7) and heap grass (3) as the separation and culture of pathogens, mold dominant, for the future to carry out census, provide the basis for the corresponding increased strain of mold strains.