论文部分内容阅读
目的:观察蛛网膜下腔注射(i.t.)ZD7288对模型大鼠痛行为的影响。方法:采用L5/L6脊神经紧结扎的方法制备大鼠神经病理痛模型,通过蛛网膜下腔插管给药。结果:ZD7288 30 g明显升高50%缩足阈值(P<0.05),表现出明显的镇痛作用,而更低剂量的15μg和7.5μg的镇痛效果不明显。在神经损伤后的5 d和14 d给予ZD7288,其镇痛效果明显好于损伤后第28 d给药(P<0.05)。这三个剂量的ZD7288并不影响大鼠的运动功能。结论:ZD7288对脊神经结扎大鼠的机械性痛敏有缓解作用,这种作用在结扎后早期比晚期更强。从行为药理学的角度证明脊髓背角超极化激活环核苷酸门控阳离子通道(HCN)可能参与了神经病理痛的形成。
Objective: To observe the effect of subarachnoid (i.t.) ZD7288 injection on the pain behavior of model rats. Methods: Rat model of neuropathic pain was established by ligating the L5 / L6 spinal nerves and was administered by subarachnoid intubation. Results: ZD7288 30 g significantly increased 50% contractile threshold (P <0.05), showing obvious analgesic effect, while lower doses of 15μg and 7.5μg analgesic effect was not obvious. The effect of ZD7288 on the 5th and 14th day after injury was significantly better than that on the 28th day after injury (P <0.05). The three doses of ZD7288 did not affect motor function in rats. Conclusion: ZD7288 can relieve the mechanical hyperalgesia in ligation of spinal nerves in rats, which is stronger in early stage after ligation than in late stage. From the perspective of behavioral pharmacology, it is proved that hyperpolarization-activated cyclic nucleotide gated cation channel (HCN) in spinal dorsal horn may be involved in the formation of neuropathic pain.