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目的:探讨同一个体食管鳞状上皮不典型增生(EDYS)和贲门腺上皮不典型增生(GDYS)组织中p53和细胞核增殖抗原(PCNA)表达特征及其意义。方法:采用免疫组化ABC法分析河南食管癌高发区38例同一个体同时发生EDYS和GDYS组织中p53和PCNA蛋白表达。结果:p53在EDYS组织中阳性率为73.0%(27/37),明显高于GDYS组织中的40.5%(15/37),P<0.01;表达有明显一致性为62.2%(23/37),Kappa=0.304,P<0.05。PCNA在EDYS组织中的阳性率为89.5%(34/38),略高于GDYS组织中的81.6%(31/38),P>0.05;表达有明显一致性为86.8%(33/38),Kappa=0.475,P<0.01。结论:p53和PCNA在同一个体EDYS和GDYS组织中存在较高的表达一致性改变,进一步提示食管鳞癌和贲门腺癌可能具有相似的发病因素和分子机制。
Objective: To investigate the expression and significance of p53 and nuclear proliferative antigen (PCNA) in the same esophageal squamous cell dysplasia (EDYS) and gastric cardia glandular dysplasia (GDYS) tissues. Methods: Immunohistochemical ABC method was used to analyze the expression of p53 and PCNA protein in EDYS and GDYS tissues from 38 same individuals in high esophageal cancer area of Henan. Results: The positive rate of p53 in EDYS was 73.0% (27/37), which was significantly higher than that in GDYS (40.5%, 15/37) (P <0.01). The positive expression rate of p53 was 62.2% (23/37) , Kappa = 0.304, P <0.05. The positive rate of PCNA in EDYS was 89.5% (34/38), which was slightly higher than that in GDYS (81.6%, 31/38), P> 0.05. The positive expression rate of PCNA was 86.8% (33/38) Kappa = 0.475, P <0.01. CONCLUSION: There is a high expression consistency of p53 and PCNA in EDYS and GDYS tissues of the same individual, which further suggests that esophageal squamous cell carcinoma and gastric cardia adenocarcinoma may have similar pathogenesis and molecular mechanisms.