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目的：研究内皮素-1对肾小球系膜细胞周期G1期进展的调控机制。方法：应用免疫组织化学、免疫印迹分析等技术检测系膜细胞在内皮素-1刺激增殖时，细胞内G1期调控蛋白cyclinD1和cdk4表达的变化。结果：正常指数式生长的系股细胞核内表达二种蛋白，在内皮素1刺激下G1期进展中cyclinD1表达增加，cdk4表达变化不明显。结论：cyclinD1是体外培养肾小球系膜细胞针对炎症因子内皮素1刺激时G1期进展的重要调节蛋白。
AIM: To investigate the regulatory mechanism of endothelin-1 on the progression of G1 phase of glomerular mesangial cells. Methods: Immunohistochemistry and Western blotting were used to detect the expression of cyclin D1 and cdk4 in mesangial cells during endothelin-1 stimulation. Results: The normal exponential growth of the nucleus expressed two proteins in the nucleus. The expression of cyclinD1 was increased and the expression of cdk4 was not significantly changed during the G1 phase stimulation by endothelin 1. Conclusion: CyclinD1 is an important regulatory protein for glomerular mesangial cells in G1 phase progression stimulated by inflammatory factor endothelin 1.