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目的:探讨西地那非对低氧大鼠心脏功能和血流动力学的影响。方法:取13只Wistar大白鼠随机分为2组:①低氧组(n=7):低氧模型是将大鼠置于模拟海拔5400m低压氧舱内3d;②低氧加西地那非组(治疗组,n=6):缺氧方法及条件同低氧组。第4d上午在模拟海拔5400m的低压氧舱内于实验前1h灌胃给予2.5mg/0.5mL枸橼酸西地那非。低氧组和治疗组动物在相应的时间点内,用10%氨基甲酸乙酯腹腔麻醉,分别经右颈外静脉插入心导管至右心室、肺动脉,用多道生理记录仪连续描记5min肺动脉压力曲线,测定心率(HR)、肺动脉压力(PAP)、右室收缩压(RVSP)和舒张压(RVEDP)、右室等容收缩期心室内压力上升最大速率和右室等容舒张期心室内压力下降最大速率(±dp/dtmax)。结果:治疗组较对照组HR、RVSP降低,-dp/dtmax增高,有非常显著性差别(P<0.01);肺动脉收缩压降低,+dp/dtmax增高,有显著性差别(P<0.05),RVEDP、肺动脉舒张压、肺动脉平均压无差别无显著性(P>0.05)。结论:西地那非具有改善急性低氧大鼠血流动力学,降低肺动脉压,保护心功能的作用。
Objective: To investigate the effects of sildenafil on cardiac function and hemodynamics in hypoxic rats. Methods: Thirteen Wistar rats were randomly divided into two groups: ① hypoxia group (n = 7): The rats in hypoxia model were placed in hypobaric chamber at 5400 m above sea level for 3 days; ② hypoxia plus sildenafil (Treatment group, n = 6): hypoxia method and conditions with the hypoxia group. On the morning of the 4th day, 2.5mg / 0.5mL sildenafil citrate was given intragastrically in the hypobaric chamber simulating the altitude of 5400m at 1h before the experiment. The rats in hypoxia group and treatment group were anesthetized with 10% urethane intraperitoneally at the corresponding time points, and then inserted into the right ventricle and pulmonary artery through the right external jugular vein respectively. The pulmonary arterial pressure Curve, heart rate (HR), pulmonary artery pressure (PAP), right ventricular systolic pressure (RVSP) and diastolic pressure (RVEDP), right ventricular isovolumic ventricular systolic pressure rise rate and ventricular diastolic ventricular pressure The maximum rate of decrease (± dp / dtmax). Results: Compared with the control group, HR, RVSP and -dp / dtmax of the treatment group were significantly increased (P <0.01); pulmonary artery systolic pressure was decreased and + dp / dtmax was increased RVEDP, pulmonary artery diastolic pressure, pulmonary artery pressure was no significant difference (P> 0.05). Conclusion: Sildenafil can improve hemodynamics, reduce pulmonary arterial pressure and protect cardiac function in acute hypoxic rats.