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LACA小鼠经60 Coγ射线全身一次照射 ,对骨髓组织切片和骨髓细胞悬液涂片 ,分别采用改良的Feulgen染色及核仁组成区相关蛋白 (AgNOR)染色 ,利用图像分析技术对在不同照射剂量、不同时间点的造血细胞核内DNA及AgNOR含量进行了定量分析。结果显示各照射组在2 5 - 7 0Gy剂量、 6h～ 4周范围内 ,骨髓均出现明显损伤及损伤后重建现象 ,且剂量越大 ,损伤越重 ,恢复亦较慢 ;其中以 5 5Gy组骨髓细胞核内DNA和AgNOR含量变化最为典型 ,在损伤早期进行性减少 ,而在恢复期出现持续性增加 ,直至恢复正常。结果提示 :骨髓造血细胞核内AgNOR及DNA含量的变化可以作为反映骨髓辐射损伤与修复程度的定量指标。
The whole body of LACA mice were irradiated with 60Coγ ray, the bone marrow slices and bone marrow cell suspension were smeared with modified Feulgen staining and nucleolar organizer region-associated protein (AgNOR) staining respectively. The effects of different irradiation doses , And the DNA and AgNOR contents of hematopoietic nuclei at different time points were quantitatively analyzed. The results showed that in the irradiated group, the bone marrow showed obvious damage and post-injury reconstruction at the dosage of 25-70 Gy and the range of 6 h to 4 weeks. The larger the dosage was, the heavier the damage was and the slower the recovery was. In the 5 Gy group The changes of DNA and AgNOR content in the bone marrow nuclei were the most typical, decreased progressively in the early stage of injury, and continued to increase in the recovery period until they returned to normal. The results suggest that the changes of AgNOR and DNA content in bone marrow hematopoietic cells can be used as a quantitative index to reflect the degree of damage and repair of bone marrow.