人yrdc基因表达促进了胃癌细胞的增殖

• 作 者: Tingsong Yang Xiaojun Shen Jia
• 机 构: Department of General Surgery,Department of Cardiothoracic Surgery
• 出 处: 中德临床肿瘤学杂志（英文版）
• 发表日期: 2004年期
• 关键词: human yrdC gene gastric carcinoma monoclonal antibody recombinant adenovirus

Objective: The aim of the research was to study the function of human yrdC gene in the gastric carcinoma cells. Methods: Human yrdC gene was isolated from human spleen tissue by RT-PCR. Anti-human yrdC monoclonal antibody was prepared by hybridoma cell technique. Recombinant adenovirus Ad.yrdC carrying yrdC gene was constructed by using the AdEasy adenoviral vector system. Recombinant adenovirus Ad.yrdCshRNA mediated yrdCshRNA was prepared by RNA interference technology. Gastric adenocarcinoma BGC-823 cells of moderate differentiation were transfected and absorbance of the transfected cells was calculated at 490 nm by methyl thiazolyl tetrazolium (MTT) method. Results: A value of the transfected Ad.yrdC group was significantly greeter than that of the non-transfected and transfected Ad.Null groups, and A value of Ad.yrdCshRNA group was significantly lower than that of the non-transfected and transfected Ad.Null groups. Con-clusion: Expression of yrdC gene has a function of promoting the proliferation of gastric carcinoma cells.