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作者用体重210±25克的大白鼠,控制光照12小时,观察了在植入后单次注射人绒毛膜促性腺激素(HCG)的终止妊娠作用。用HCG制剂,效能约为3000国际单位/毫克,溶于生理盐水并用生理盐水稀释后,在妊娠不同天的15点做颈部背侧肌肉注射。为观察孕酮对HCG处理鼠妊娠的保护作用,从妊娠的第7或第8天到妊娠14天,每天肌肉注射两次溶于芝麻油的孕酮。在与雄鼠合笼过夜受精后,当发现阴栓或阴道涂片中出现精子时,算作妊娠的第1天。在妊娠第15天用氯仿处死动物,记录正常及吸收的胎数。结果表明,在妊娠第8天单次注射不同剂量(50—500单位/鼠)的HCG,在所有动物都观察到
The author used a mouse weighing 210 ± 25 grams to control light for 12 hours and observed the termination of pregnancy by a single injection of human chorionic gonadotropin (HCG) after implantation. HCG preparations, the potency of about 3000 international units / mg, dissolved in saline and diluted with saline, 15 days in pregnancy on the back of the neck dorsal muscle injection. To observe the protective effect of progesterone on HCG-treated mice, intramuscular injection of progesterone dissolved in sesame oil twice daily from the 7th or the 8th day of gestation to 14 days of gestation. After overnight cocooning with male rats, the first day of pregnancy is counted when sperm are found in the thrombus or vaginal smear. Animals were sacrificed on day 15 of gestation with chloroform and the number of normal and absorbed fetuses was recorded. The results showed that a single injection of different doses (50-500 units / mouse) of HCG on day 8 of gestation was observed in all animals