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通过分析稻瘟病菌Rho GAP家族基因与几丁质合酶家族基因的表达关系,研究该家族基因对细胞壁形成的影响。利用不同生物信息学软件对Rho GAP家族进行序列分析,运用RT-PCR技术获得了该家族基因的部分c DNA序列,通过实时荧光定量技术分析Rho GAP基因与几丁质合酶基因的表达关系。结果表明,稻瘟病菌数据库中8个Rho GAP基因均含有保守的GAP结构域,且为水溶性蛋白,蛋白分子量相对较大,系统进化分析结果显示,16个Rho GTP酶激活蛋白可分为两类;不同的几丁质合成酶基因在不同Rho GAP基因突变体中的表达量不同,总的趋势是上调的。说明Rho GAP基因家族蛋白可能参与了稻瘟病菌不同阶段细胞壁的合成,本结果为进一步研究Rho GAP家族基因参与多样性的代谢途径奠定了基础。
By analyzing the expression of Rho GAP family genes and chitin synthase family genes in Magnaporthe grisea, the effects of this family of genes on cell wall formation were investigated. The Rho GAP family was sequenced by using different bioinformatics software. The partial c DNA sequence of this family gene was obtained by RT-PCR. The relationship between Rho GAP gene and chitin synthase gene expression was analyzed by real-time fluorescence quantitative analysis. The results showed that all of the eight Rho GAP genes in the M. grisea database contained a conserved GAP domain, which was a water-soluble protein with a relatively large molecular weight. Phylogenetic analysis showed that 16 Rho GTPases were divided into two ; Different chitin synthase genes in different Rho GAP gene mutant expression levels are different, the general trend is up-regulated. This indicated that the Rho GAP gene family protein may be involved in the cell wall synthesis of different stages of Magnaporthe grisea. The results laid the foundation for further study on the metabolic pathway involved in diversity of Rho GAP family genes.