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本文以ＱＰＣＲ 方法检测了２３ 例与乙肝相关的肝细胞癌和４０ 例慢性乙型病毒性肝炎病人的血ＨＢＶＤＮＡ含量。肝细胞癌组与慢性乙型肝炎组ＨＢＶ ＤＮＡ 定量分别为１０８－８４ ±２－９６ 和１０６－９１ ±１－５６ 拷贝／ｍｌ；两者差异显著（ Ｐ＜ ０－０５） ；两类慢性乙肝病人血清ＨＢＶＤＮＡ定量检测结果分别为１０６－４８ ±１－３２ 和１０７－１４ ±１－８５） 拷贝／ｍｌ，病人血清ＨＢＶ ＤＮＡ 含量无显著差异（ Ｐ＞ ０－０５） 。结果提示：与乙肝相关的肝细胞癌存在高水平的ＨＢＶＤＮＡ复制。
In this paper, the HBV DNA content of 23 cases of hepatocellular carcinoma associated with hepatitis B and 40 cases of chronic hepatitis B were detected by QPCR. The quantification of HBV DNA in hepatocellular carcinoma and chronic hepatitis B groups was 108-84 ±2-96 and 106-91 ±1-56 copies/ml respectively; the difference was significant (P < 0-05); two types of chronic hepatitis B The serum HBVDNA levels in the patients were 106-48 ± 1-32 and 107-14 ± 1-85 copies/ml, respectively, and there was no significant difference in serum HBV DNA levels (P> 0-05). The results suggest that there is a high level of HBV DNA replication in hepatocellular carcinoma associated with hepatitis B.